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Berkeley Lab Currents

October 9, 1998


Semiconductor Nanocrystals Serve As Fluorescent Probes in Biological Labeling

By Lynn Yarris

Some of the more shadowy secrets of biology may soon be illuminated through the use of a new type of fluorescent probe developed by scientists at Berkeley Lab.

A joint LBNL-UC Berkeley research team led by Paul Alivisatos and Shimon Weiss has announced the development of nanometer-sized crystals of semiconductors, such as cadmium selenide and cadmium sulfide, that can be used as fluorescent probes for the study of biological materials. These semiconductor nanocrystals offer a distinct advantage over conventional dye-molecules in that they emit multiple colors of light, which means they can be used to label and measure several biological markers simultaneously.

The unique optical properties of these semiconductor nanocrystals also hint at the possibility of observing changes that take place in labeled biological systems, such as living cells, over a period of time. This research was reported in the Sept. 25 issue of Science.

Alivisatos is a leader in the production by chemical means of semiconductor nanocrystals, simple inorganic solids consisting of a hundred to a hundred thousand atoms. He holds a joint appointment as a senior staff scientist with the Materials Sciences Division (MSD) and as a professor of chemistry at UC Berkeley. Weiss, a staff scientist with MSD, is an authority on single molecule fluorescence and spectroscopy. He approached Alivisatos with the proposal for this joint study.

Other members of the team who co-authored the paper in Science were Marcel Bruchez Jr., who also holds a joint LBNL-UCB appointment, and Mario Moronne and Peter Gin, with the Life Sciences Division.

"Form follows function" is the golden rule in cell biology, which is why microscopy has been the heart and soul of this research field and fluorescent-labeling, one of its most widely used tools. In fluorescent labeling, markers, usually antibodies that attach themselves to specific proteins, are tagged with dye-molecules that fluoresce, or emit a specific color of light when stimulated by laser light, usually from a confocal microscope.

"Sometimes, in order to fully characterize a sample, a population of cells for example, you need to look at combinations of markers," says Alivisatos. Such measurements require multiple-color light emissions which are difficult to obtain with conventional dye molecules.

"Ideal probes for multi-color experiments should emit at spectrally resolvable energies, should have a narrow, symmetric emission spectrum, and the whole family should be excitable at a single wavelength," the authors of the Science paper wrote.

Semiconductor nanocrystals met these demands in a "dual emission from single excitation" labeling experiment on mouse tissue cells called 3T3 fibroblasts. A core nanocrystal of cadmium selenide was enclosed within a shell of cadmium sulfide to boost the amount of fluorescence and reduce photochemical degradation. This core-shell combo was then enclosed within a shell of silica for water solubility and biocompatibility.

With earlier work by Alivisatos having shown that the color of light emitted by a semiconductor nanocrystal depends upon its size, the mouse cells were labeled with two different sizes of core-shell nanocrystals. It was also known that modifying the surface of the silica shell can be used to selectively control its attachment to components within a cell. In this case, the smaller nanocrystals (two nanometers), which fluoresced green, were modified to penetrate the nucleus of each cell, and the larger nanocrystals (four nanometers), which emitted red light, were modified so that they would attach themselves to actin filaments along the outer cell membrane.

Using wide-field microscopy, the green and red labels were clearly visible to the naked eye and could be photographed in true color with an ordinary camera. Confocal microscopy images showed that cell nuclei had been penetrated with the green probes and the actin fibers had been stained red. After repeated scans, the nanocrystal labels showed far less photobleaching than would occurred in the control sample labeled with conventional dye molecules.

"The development of semiconductor nanocrystals for biological labeling gives biologists an entire new class of fluorescent probes for which no small organic molecule equivalent exists," the authors of the Science paper wrote. "These nanocrystal probes can be complementary and in some cases may be superior to existing fluorophores."

The authors also assert that, compared with conventional fluorophores, semiconductor nanocrystals have a "narrow, tunable, symmetric emission spectrum, and are photochemically stable." These features, along with a relatively long fluorescence lifetime (hundreds of nanoseconds), indicate that in addition to serving as direct probes, semiconductor nanocrystals could also be used as "sensitizers" for traditional dye-molecule probes, meaning they transfer their excitation energy to the dye-molecule.

An earlier research team led by Weiss demonstrated that this energy sensitizing phenomenon, known as fluorescence resonance energy transfer (FRET), when it takes place between a single donor and a single receptor, could allow for the labeling and observation of dynamic events such as conformational changes in a protein.

"The use of semiconductor nanocrystals should allow us to do unique FRET experiments," says Weiss. "For example, labeled molecules could be made to emit different colors at different times of an event."

Weiss and Alivisatos and their co-authors also believe that semiconductor nanocrystals could be applied to x-ray- and electron-based imaging techniques, and could serve as tunable infrared dyes for detecting fluorescence in, among other things, blood samples.

Photos: Materials Sciences' Paul Alivisatos (above) and Shimon Weiss (right) led the team that developed semiconductor nanocrystals for use as fluorescent probes. Photos by Roy Kaltschmidt (top: XBD9810-02448.jpeg, bottom: XBD9810-02450.jpeg)

Photos: Mouse 3T3 fibroblasts. (probe.jpeg)


Berkeley Mayoral Forum at Lab Oct. 14

Four of the five candidates for Mayor of Berkeley in this November's election will participate in a brown bag forum at noon on Wednesday, Oct. 14, in the Bldg. 50 auditorium.

The event was scheduled because of the increasing attention the city has paid to the operations of Berkeley Lab. According to the Lab's economic impact analysis, more than 1,000 of the Laboratory's employees live in Berkeley.

The candidates--incumbent Shirley Dean, Michael Delacour, Don Jelinek, and Robert Krumme, will each be given seven minutes to make a statement, and then will take questions from the audience. The event will be moderated by Reid Edwards, Berkeley Lab's manager of government relations. All Laboratory employees are encouraged to attend.

Turn to Page 3 for information on the participating candidates.


Integrated Safety Management: A Better Plan

By Monica Friedlander

It was not so long ago that representatives from the Department of Energy would come to the Laboratory once a year on safety checks, bringing along a fat checklist. Say, for example, the fire engineer inspected the Firehouse. What he would look for was the number of sprinklers, the placement of fire doors, the number of people in the department. Three guys on the engine--check. Fire door here missing--no check. That's how Dick Nolan, DOE's Berkeley site manager, remembers the old way of going about EH&S business, one that resulted into what he calls a "checklist mentality."

Not anymore. What the DOE and the Laboratory focus on now is results. Instead of dictating a safety protocol to the Lab, the DOE makes sure the performance is up to par year-round. In the case of the Fire Department, that means assessing actual response time in an emergency situation. "It's things that make sense," Nolan says. "We shifted from a compliance mentality to what we call operational awareness. And instead of coming once a year to do it, we're engaged with the Laboratory the whole year. The Laboratory has opened up to us."

Over the last few years, representatives of Berkeley Lab and the DOE have worked closely together to develop the Laboratory's recently-implemented Integrated Safety Management (ISM) Plan, which reflects an entirely new approach to safety in the workplace. Instead of safety being seen as a separate function, distinct from all other activities at the Laboratory, the new plan seeks to incorporate safety into the larger picture. And for all its daunting title, the Integrated Safety Management Plan is actually based on a very simple, common sense principle: integrate safety awareness and planning into day-to-day business.

"Integrated safety management starts from a philosophy of looking at the work that we're doing," says David McGraw, head of the Lab's Environment, Health and Safety Division. "As we design the work, we design right into it the EH&S requirements. We want to build a habit for the individual investigators to think in a different way about safety: to think about it the way they think about their work and their research. This approach allows us to tailor the requirements to the work being done."

The concept may sound very simple, but it took the DOE a long time to reject the traditional, one-size-fits-all approach to EH&S. The change, Nolan says, was brought about both by a shift in industry practices and by a new direction from Washington, as demonstrated in Vice President Gore's Government Performance and Results Act.

"That has encouraged a revolution in government focusing on results," Nolan said. "In many respects that philosophy is reflected in ISM. Instead of safety being a stand-alone function that somebody else does, that EH&S does, it is something that you're responsible for."

In practical terms, ISM means that safety considerations are to be included in project planning just like all other elements, such as budget considerations or choice of equipment. Says McGraw: "My goal is to get to a place in EH&S where you don't think about it. Safety awareness becomes second nature."

ISM is based on seven guiding principles that provide a framework for the plan, guide its implementation and provide test points for evaluating its effectiveness. Central to these are "line management responsibility" and "clear roles and responsibilities."

"In final analysis," Nolan says, "somebody should be fired if there are serious safety deficiencies that are not getting corrected. And the person getting fired should be clearly identified."

Other principles include competence commensurate with responsibility, balanced priorities, identification of EH&S standards and requirements, hazard controls tailored to work being performed, and operations authorizations.

On one hand, this approach seeks to decentralize responsibility for safety in the workplace, with the divisions taking on an increasingly greater share of it. At the same time, McGraw says, EH&S staff will go out in the field more, particularly in the initial stages of implementation, to spend time partnering with researchers and providing them with the resources and expertise to help them build safety into their day-to-day work.

"I hope this will improve the respect that researchers have for EH&S professionals, and vice versa," he said.

This mutual respect was evident from the start of the process, Nolan says. As the ISM Plan was being developed last fall, the DOE team and Lab managers worked together on division safety plans. "That shows a high degree of trust between the Laboratory and DOE," he said. "It's unprecedented, to my knowledge, for a Laboratory to open up its internal processes by inviting the DOE to review division plans."

McGraw concurs, praising the way division directors have stepped up to the challenge and been personally involved in the development of the plans. "The response was much better than I could have hoped for," he said. "Not only has each division director written a customized division safety plan, but defended it in front of management."

Just because the new plan is in place, however, does not mean that the bulk of the work is done. In fact, both Nolan and McGraw caution, the real challenges still lie ahead: adjusting to the new way of doing business and establishing measurements to ensure the plan is being effectively implemented. "People are going to have to change their way of thinking," McGraw says. "And that's very hard for people to do. It's the most difficult single impediment I see."

For now the logistics are in place. The plan has been endorsed at the highest levels of management, both at the Laboratory and at the DOE. It has been embraced at the division level and is now filtering down into operations. And EH&S professionals continue to provide operational divisions with the expertise needed to comply with the new vision. What's left is for the plan to stand the test of time and turn this overarching vision into reality.

"Development of this program has given the Laboratory as well as our local operations office a lot of visibility," says Nolan. "It is looked upon as a model. I'm proud of that, and the Laboratory should be very proud. Now we just need to carry through and make sure it works. We're off to a good start. We're on our way."


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News Digest


Washington Report

Energy Research Office Renamed, Gets Funding Boost

Under the energy and water appropriations bill approved by Congress last week, DOE's Office of Energy Research will be renamed the Office of Science and will receive a 16 percent boost in funding in FY99. Martha Krebs, director of the office, says that the extra funding shows Congress understands the value of research activities at DOE.

The bill provides $2.6 billion in FY99 for science programs at DOE, compared to the Administration's request of nearly $2.5 billion and the $2.2 billion approved in FY98. President Clinton must sign the bill to make it official.

A major beneficiary of the additional funding is the Spallation Neutron Source (SNS), the world's largest neutron-scattering device, scheduled for completion in 2005 at Oak Ridge National Laboratory. House and Senate conferees agreed to provide $130 million for SNS. While that is $40 million below DOE's request, it is $30 million above the level the House version of the bill would have provided.

Krebs believes the $40 million difference can be recouped in later years, and said that Congressional approval of a significant amount of funding for SNS, DOE's single biggest project for the foreseeable future, shows appreciation for the value of large-scale research.

"We're back in the business of building big facilities," Krebs says.

--Lynn Yarris


Stanford Beats Cal, But Harvard Leads Them All

Harvard University finished first in the latest ranking of U.S. research universities, according to the Institute for Scientific Information in its latest issue of ScienceWatch. Harvard not only churned out more papers than any other university between 1993 and 1997, but the work was rated as having higher scientific impact across the board. Stanford finished second and UC Berkeley seventh.

To rank the top 100 federally funded research universities in 21 separate fields, ScienceWatch worked out the average number of times that papers from researchers at each institution were cited in another paper. These scores were then calculated as a percentage above or below the world average for papers in the same field, to yield an estimate of their "relative impact."

Harvard placed in the top 10 in 17 of the 21 categories, ScienceWatch reports. It was followed by Stanford, (13 top-10 placings), Caltech (11), Yale (9), University of Michigan (9), MIT (8), UC Berkeley (7), University of Washington (6), UC Santa Barbara (6), Cornell (6), and UC San Diego (6).


President's Science Advisor Visits Lab

Photos: Neal Lane (middle), Assistant to the President for Science and Technology Policy and director of the Office of Science and Technology Policy, toured a genome sciences lab during his visit to Berkeley Lab on Sept. 30.

Lane met with Lab Director Charles Shank (shown here on the left) and Life Sciences' Gerry Rubin(right), head of the Drosophila Genome Project. The visit included discussions about the role of DOE labs in human genome projects.

Lane served as director of the National Science Foundation from 1993 until moving to his current position as presidential science advisor this year. Photo by Roy Kaltschmidt (XBD9810-02452.jpeg)


Director Shank Praises Laboratory Progress In Testimony Before Congress

Berkeley Lab Director Charles V. Shank has testified before Congress that the U.S. Department of Energy and its federal laboratories have made significant progress in improving efficiencies and unlocking bureaucratic logjams since 1995.

In comments presented on Sept. 23 to the Subcommittees on Basic Research and Energy and Environment of the House Science Committee, Shank cited an atmosphere of trust and a commitment to partnerships as prime reasons why operations have been more effective and efficient. He was among several witnesses addressing reforms that have been implemented within the DOE since the so-called "Galvin Report" criticized various aspects of the department's management three years ago.

Using Berkeley Lab's experience as a model, Shank reviewed several examples of improved productivity through the elimination of costly administrative systems, unnecessary prescriptive oversight, and diffusion of responsibility.

"The growing level of trust and mutual respect between [the DOE and the laboratories] has resulted in very positive results that have saved the nation's taxpayers many millions of dollars," he told the panel. At Berkeley alone, he noted, "the combined effort in streamlining, outsourcing, and administrative systems updating resulted in a reduction in our composite overhead rate of 17 percent."

Shank cited assistance received from DOE's Oakland site office that allowed Berkeley Lab to gain access to low-cost federal power from the Western Area Power Administration, saving $2.85 million since 1995. A streamlined procurement system resulted in $500,000 savings in fiscal 1998 alone.

Shank said the new working relationships have also improved operating activities, leading to the implementation of the Integrated Safety Management and Work Smart Standards programs, the Nuclear Regulatory Commission pilot on external regulation, and more effective contract management. Efficiencies achieved in the National Environmental Policy Act "significantly reduced the number of steps and amount of paperwork passed from office to office." Streamlined support services, through outsourcing and systems updating, allowed an 18 percent reduction in personnel supporting the overhead budget, increasing the ratio of scientific staff to support staff at Berkeley, he added.

At the same time, "we have also increased our investment in infrastructure improvements," resulting in "a 50 percent reduction in the annual backlog of plant maintenance work, and a corresponding reduction in demands for future infrastructure projects," Shank said in his testimony.

He pointed out several examples of the DOE's more effective use of its national laboratories through partnerships and primary competencies: the Joint Genome Institute, combining the talents of Berkeley, Livermore and Los Alamos scientists; the Spallation Neutron Source, to be located at Oak Ridge; and the B-Factory project at the Stanford Linear Accelerator Center.

"In each of these projects," Shank said, "DOE is taking advantage of the unique strengths and capabilities of each institution. This is a far more efficient process than building up new teams at each site for each new project."

Shank concluded by saying, "We are working in greater partnership among the laboratories and with the DOE to insure that our laboratories remain focused on their missions, maintain their core competencies and infrastructure, and conduct their business in a cost effective manner."

Congresswoman Barbara Lee (D-Oakland), a member of the House Science Committee who welcomed Shank to the hearing, acknowledged in a prepared statement that "Berkeley Lab has incorporated many aspects of reform in its current operations and has developed a mutual relationship with the DOE located in Oakland to pursue substantive measures to ensure effective and efficient management.

"One of the things that has been encouraging," she said, "is the Lab's effort to reach into the community to utilize local people, business and agencies with which to contract to implement some of the functions of the Lab."--Ron Kolb


Mayoral Candidates in Lab Spotlight

Four of the five candidates running for mayor of Berkeley--incumbent Shirley Dean, Michael Delacour, Don Jelinek, and Robert Krumme--are scheduled to participate in a lunch-time brown bag forum to be held on Wednesday, Oct. 14, in the Bldg. 50 auditorium.

The candidates will make a statement and take questions from the audience. Reid Edwards, Berkeley Lab's manager of government relations, will moderate the event. All laboratory employees are encouraged to attend.

Shirley Dean

Mayor of Berkeley since 1994, Shirley Dean was born in Colorado and has been a resident of Berkeley for the past 46 years. She was recently elected chair of the East Bay Public Safety Corridor Partnership and serves on the Executive Committee of the Alameda County Conference of Mayors. Dean was elected to the Berkeley City Council in 1975 and reelected in 1979. She left the Council in 1982 and was elected and repeatedly reelected as representative of District 5 in North Berkeley in 1986. A UC Berkeley alumni, Dean works part-time in UC's Office of Undergraduate Admission and Relations with Schools. She serves on numerous boards and committees.

Photos: Shirley Dean

Michael Delacour

Self-described as a socialist and "an alternative candidate for mayor of Berkeley," Delacour moved to Berkeley from Southern California 33 years ago. He is active in union and community activities and was elected steward and delegate to Boilermaker Local 549.

Photos: Michael Delacour

Don Jelinek

A lawyer, Jelinek served on the Berkeley City Council from 1984 to 1990. He moved to Berkeley in 1968 and started his own law firm three years later. Previously he was involved in the civil rights movement in the South. He and his wife Jane Scherr live in the South Shattuck neighborhood of Berkeley.

Photos: Don Jelinek

Robert Krumme

A self-employed engineering consultant running as an independent, Krumme serves on the Citizen's Budget Advisory Committee and on the Public Works Commission. He was born in Wisconsin and earned his bachelor's degree from Princeton University. He and his wife Ileana reside in North Berkeley.

Photos: Robert Krumme


Assistance Available with New Parking Permit Application

The Site Access Office has announced that computers will be set up during lunchtime the week of Oct. 12, both at the cafeteria and in Bldg. 65, to help employees enter their data for the new parking permit application. Personalized service will be offered to anyone who needs assistance using the form.

New parking permits will be issued during the month of October, and all employees need to fill out the online application
( to obtain permits by the Nov. 1 deadline. All previous parking permits will become invalid after that date (with the exception of motorcycle decals) and should be removed from vehicles.

The new permits will be distributed starting Oct. 19. Employees will be notified by their division personnel when the permit is ready for pickup. A driver's license, the old parking permit and the Lab ID will be required for identification purposes.

All questions regarding parking permits should be directed to Sue Bowen, [email protected]. Information may also be found on the website listed above.


Currents Readership Survey

Please take a few minutes to complete the following survey to help us evaluate Currents' effectiveness. Your responses will allow us to make changes that will better serve your needs and interests. Please cut out the survey and mail it by Friday, Oct. 16, to the address listed below. Thank you.


Around the Lab


FY 1999 Laboratory R&D Program Awards Announced

LDRD FY99 Allocations

Sept. 9, 1998

Lab Director Charles Shank has announced the selection of 73 projects for support by the Laboratory Directed Research and Development (LDRD) Program for Fiscal Year 1999.

A total of about $9.2 million in both operating expenses and capital equipment has been allocated for LDRD. One hundred seventy-one proposals were submitted requesting a total of $27.1 million in response to the Call for Proposals issued earlier this year (see Currents, March 6, 1998). The proposals were evaluated in a review process that used the scientific judgment and priorities of the division reviews and the director's review committees.

"The LDRD program is a primary mechanism to support new ideas as well as strengthen Berkeley Lab in important stra-tegic directions," Shank said.

"This year we had an abundance of riches to choose from, with the result that this was also one of our most competitive years. Among the projects funded, key strategic directions included computational proposals, research and development at the Advanced Light Source, and in the physical biosciences area."

Proposals funded included cross-divisional, multi-investigator, and individual investigator projects. Director Shank expressed his appreciation to all principal investigators who submitted proposals for their effort and creativity.


Lab Launches Charity Campaign

This November the Laboratory is reviving its annual charity giving campaign with a revised version called Berkeley Lab SHARES (Science, Health, Academics, Resources, Education and Services). Lab employees will receive their information packets by the end of October and will be able to choose the charity of their choice.

"As one of the world's premier scientific research organizations, the Laboratory has an outstanding record of contributing to the betterment and understanding of our world," said Laboratory Director Charles Shank. "Berkeley Lab SHARES gives you the opportunity to contribute as an individual to any of hundreds of non-profit organizations, which also seek to enhance all of our lives, whether by helping our communities, protecting natural resources, or supporting health-related research."

Berkeley Lab SHARES will allow employees to pick from literally hundreds of non-profit organizations represented by three umbrella organizations: United Way Bay Area, EarthFirst of California, and Combined Health Appeal. Organizers of the campaign have also chosen to include 19 Bay Area organizations which have links to the Laboratory and its mission.

Employees who wish to participate should return their forms in the preaddressed envelope by Friday, Nov. 20. Donations can be made either by enclosing a check or opting for payroll deductions, which woud take effect starting in January 1999.

Over the next few weeks, Currents and Headlines will feature more information about the campaign, the participating organizations and those who ultimately benefit from them. On Wednesday, Oct. 28, a fair will be held from 11:30 to 1:30 in or outside the cafeteria, offering additional information about the United Way, Combined Health Appeal and EarthFirst California organizations and their member agencies. Also attending will be several East Bay organizations supported by the Lab's campaign.

More information about the SHARES campaign will soon be available on the web at

Photos: hands.tif


New Employees

David J. Aston, DIRC
Dennis J. Baum, ENG
Carrie L. Butenhoff, LSD
Willow B. Dean, LSD
Laura L. Eichman, ICSD
Eric H. Esarey, AFRD
Tony J. Genovese, COMP
Terrence L. Hamilton, OPER
Daniel S. Hawkes, ICSD
Michael G. Henseler, ASD
Sui Hing Jen, HR
Kary M. Moulden, ICSD
Erik S. Richman, ICSD
Henry P. Sartorio, DIRC
Jeff F. Shreve, LSD
Raymond H. Statam, EH&S
Marie A. Sticht, ASD
Jerome L. Turner, ICSD
Ryan P. Van Luchene, LSD
Charlene K. Woolcott, ASD


Bulletin Board


Hearst Avenue Construction Will Disrupt Traffic

Repaving of Hearst Avenue began on Oct. 5 and will continue through Dec. 15. Construction will affect the area between Oxford Street and Highland Place. Traffic flow will be reduced to one eastbound and one westbound lane on either side of the street for the duration of the project. Traffic flow between Euclid Avenue and Gayley Road will be one way eastbound during the first phase of the project, with detours expected. Commuters are encouraged to use alternate routes to avoid congestion and delays.


Blackberry Canyon Construction Update

Facilities has announced that construction of underground electrical ducts for the Blackberry Canyon Switching Station project is about 40 percent complete, with the bulk of the work finished on McMillan Road. Construction has now moved to Cyclotron Road between Bldg. 71 and the Bevatron Circle--the final segment of work involving Lab roads. Completion is expected by Oct. 30. Until then, one-lane traffic control with flaggers will be used. For further information call project manager Chuck Taberski at X6076.


Runaround: Last Call

The annual Berkeley Lab Run-around will be held next Friday, Oct. 16. All employees are encouraged to participate. The Runaround starts at the Firehouse and ends at the cafeteria parking lot. A downloadable map of the course can be found on the web at For more information contact Steve Derenzo (SEDerenzo@lbl. gov) or look up the Runaround website at the URL above.


Retirement Plaques

The Engineering Technology Department offers Lab employees the opportunity to purchase retirement plaques. Cost information and an order form can be found on the Lab's home page under Laboratory Support Services, Retirement Plaques (or


New E-mail for Health Services

Please note that Health Services has changed its e-mail to healthservices@


Scoopfuls of Fun in Computing Sciences

Photos: Bill McCurdy the Ice Cream Man--aka director of the Computing Sciences Division--was among the CS senior managers dishing out frozen delectables during the division's second annual ice cream social, held on Sept. 21 on the cafeteria patio. Photo by Roy Kaltschmidt (XBD9809-02402.tif)


Flu and Pneumonia Shots

Health Services will once again offer low-cost flu and pneumonia vaccinations to employees 18 and older from 8 a.m. to noon on consecutive Thursdays, Oct. 15 and Oct. 22, in Bldg. 26. The vaccination program is made possible through the Visiting Nurse Association and the Hospice of Northern California (VNAHNC). The cost is $10 for the flu vaccine and $25 for the pneumonia vaccine, payable by check that day to VNAHNC.

People encouraged to consider vaccination include those with heart, lung or other serious health problems and persons over the age of 65. To make your appointment call X6266.

Health Services suggests that you contact your personal physician for any questions regarding the vaccine.


Discover Magazine Awards for Technological Innovation

Discover Magazine is accepting nominations for its tenth annual award program recognizing exceptional technological breakthroughs. The nomination deadline is Nov. 13. Categories include aerospace, communications, computers, emerging technologies, energy, environment, imaging, materials, medical diagnostics, robotics, sound, transportation, and video, among others. The Lab's Technology Transfer Department is coordinating the submissions. Forms and information may also be found on the magazine's website at To receive a nomination package or for further information, contact Bruce Davies (X6461, [email protected]) or Steve Hunter (X5366, [email protected]).


Volunteers Needed for Biomedical Research Studies

Healthy adults over the age of 21 are sought to participate in one of two studies investigating the effects of respiratory motion on Positron Emission Tomography (PET) images of the chest. The goal of the studies is to improve the quality of PET imaging and our understanding of disease processes.

These studies are not expected to result in any medical benefit or harm to the subject. Each participant will be interviewed by the principal investigators regarding their health history and will be given a verbal explanation of the procedure and any possible discomforts.

Individuals who are not in good health, pregnant women, or anyone with a history of respiratory problems should not volunteer. No monetary compensation will be offered. For more information contact Thomas Budinger at X5435.


Two-Day Blood Drive

The next blood drive at Berkeley Lab is scheduled for Oct. 22 and 23 from 7 a.m. to 1 p.m. in the Bldg. 70A conference room. The last blood drive at the Lab attracted a record 146 donors, and the Blood Bank of Alameda-Contra Costa Counties hopes this effort will be at least as successful. Donors are urged to make an appointment by calling X4009. For more information or to register, contact Helane Carpenter at X4009 or [email protected].


New Course on Infant & Child CPR

Instruction on infant and child cardiopulmonary resuscitation (CPR) will be offered on Wednesday, Oct. 21, from 5:30 to 7:30 p.m. in Bldg. 48-109. The class will be taught by Lab Fire Department personnel. Class fee is $10 and enrollment is limited to 10 people. Participants must have taken adult CPR and are encouraged to bring their Heartsaver CPR manual to class. To enroll, contact Susan Aberg at X7366.


What's New at TEID

The Lab's Technical and Electronic Information Department will be presenting "What's New in TEID: Services and Samples" in the cafeteria lobby during lunchtime on Oct. 21-23. The event will feature video, photo and library services. TEID staff will be available to answer questions about technical reports, brochures, presentations, online information systems, web pages, and its free consulting services. TEID is a part of Computing Sciences and serves the Lab's communication and information management needs.


Change Your Smoke Detector Battery

October is fire prevention month and the Lab's Fire Department would like to remind you to change your batteries in your smoke detectors. A good way to remember this is to change the battery when you change your clocks back to standard time


Golf Club Results

Results from Indian Valley Golf Club tournament:

Flight 1
1. Henry Rodriguez
2. Ralph Sallee
3. Mark Campagna
4. Gilbert Goo
5. Craig Pedrey

Flight 2
1. Harry JeLonek
2. Ed Miller
3. Don Weber
4. Ron Gervasoni
5. Reba Rodriguez



General Interest

October 9 - 23, 1998

MON., OCT. 12

Noon, cafeteria and Bldg. 65

WED., OCT. 14

Noon, lower cafeteria


8 a.m. - noon, Bldg 26

FRI., OCT. 16

Noon, starts at Firehouse

WED., OCT. 21

Noon, cafeteria

5:30 - 7:30 p.m., Bldg. 48-109


7 a.m. - 1 p.m., Bldg. 70 conf. rm.

8 a.m. - noon, Bldg 26

Noon, cafeteria

7:30 a.m. - 3:30 p.m., Bldg. 54 parking lot

FRI., OCT. 23

Noon, cafeteria

7 a.m. - 1 p.m., Bldg. 70 conf. room

Items for the calendars may be sent via e-mail to [email protected], faxed to X6641 or mailed to Bldg. 65B. The deadline for the Oct. 23 issue is 5 p.m. Monday, Oct. 19.


Seminars & Lectures

MON., OCT. 12

"Localization and Population Biology" will be presented by David R. Nelson of Harvard.
4:30 p.m., 1 LeConte.
Tea at 4 p.m., 375 LeConte

TUES., OCT. 13

"Monitoring Field-Scale Bioremediation of Wood Preservative Contaminated Soil in Solid-Phase Reactors: Biological Processes, Biostimulation and Health-Risk" will be presented by Ronald Sims of Utah State University.
Noon, Bldg. 50A-5132

"Measurement & Modeling Implications of Transfer and Transformation Processes at the Plant/Air Interface" will be presented by Randy Maddelena of EET.
Noon, Bldg. 90-3148

"Signaling by SRC & RAS" will be presented by G. Steven Martin of UC Berkeley.
4 p.m., Bldg. 66-316


"Current Findings in Commercial Duct Leakage and Saling" will be presented by Woody Delp, Mark Modera and Karl Brown of EET.
Noon, Bldg. 90-3148

MON., OCT. 19

"New Insights from the Magnetic Fields of Planets & Satellites" will be presented by David Stevenson and George Van Osdol of Caltech.
4:30 p.m., 1 LeConte. Tea at 4 p.m., 375 LeConte

WED., Oct. 21

"Women in Physics: Pleasures and Problems" will be presented by Dharam V. Ahluwalia of Los Alamos.
10:30 a.m., Bldg. 50 auditorium


"Simulation as a Tool for Performance Validation" will be presented by Tim Salsbury of the EET Division's Indoor Environment Department.
Noon, Bldg. 90-3148

"Ballistic Electron Emission Microscopy for Au & CoSi2: A Theoretical Discussion" will be presented by F. Flores Sintas of Universidad Autonoma de Madrid, Spain.
1:30 p.m., Bldg. 66 auditorium

FRI., OCT. 23

"The Equations of Motion of an Electron" will be presented by Andy Sessler of the Center for Beam Physics.
10:30 a.m., Bldg. 71 conf. rm.


October EH&S Class Schedule


Lab Life


Thanks for "Waves to Wine" Contributions

Marek Szajbler would like to thank all Lab employees who generously contributed to the "Waves to Wine" fundraising event for the National Multiple Sclerosis Society. Szajbler participated in the event during the last weekend in September, biking a total of 175 miles over two days and raising $1,672 for the Multiple Sclerosis Society, of which $1,120 came from Lab supporters. A total of $455,000 was raised that weekend.

For information on next year's event or any other questions, contact Szajbler at X5029, ESzajbler@

Photo: Marek Szajbler (biker.tif)


Flea Market

Autos / Supplies

'79 TOYOTA Celica, 156K, 5 spd, ac, sun roof, exc tires, new clutch, recent tune-up, well maintained, all records, very reliable, registered until 10/98, $1,000, X5707, Raul

'81 HONDA Accord LX, hatchback, pwr steering, 5 spd, Pioneer stereo, Yakima roof rack, exc running cond, well maintained, great little car for visiting scholar, $1,200/b.o., David, X4975, 528-4268

'83 TOYOTA, Long Bed Toy pickup, 173K mi, $1,999, infirm, Warren, X7964, 531-1726

`84 FORD F150, half ton truck, white, 4 wd, short bed, camper shell, trailer hitch, 6 cyl, 4.9 l, 150K mi, eng & other parts replaced, smog, brakes renovated, great car for the outdoors $3,300/b.o., Jan, 642 4359

`86 TOYOTA Tercel Deluxe, 4 dr hatchback, 86K mi, navy blue, am/fm, new timing belt, spark plugs, ignition rotor, fuel & air filters, tires, inner & outer bearings, well maintained, clean, ac needs recharging, $2,800/b.o., Ashok, X4651, 237-8806

'88 SUBARU DL FWD Sedan, 100K mi, silver, stereo, new brakes, battery & tires, good cond, $ 1,400, Susanne, 642-5589 (work), 845-3605 (eve)

'91 Geo Metro LSi Convertible 2 dr, blue, 80 mi, 5 spd, am/fm cassette, ac, $3,200, Chaincy, X6762 or 795-7552

`94 FORD Mustang, silver, 57K mi, new transmission, new carpet, fully loaded, pwr steer, pwr windows, ac, anti lock brakes, pwr seat, air bag, cd & cassette, runs great, $9,500, Lisa, X5314, (925) 906-9786

'95 JEEP Cherokee Sport, Command-Trac 4WD, red, exc cond, 4 dr, 5 spd manual, ac, driver air bag, am/fm/cassette, pow tilt/ steering, roof rack, new tires, full size spare, 85K mi, $11,000, Edith, X5553, 222-6385

`96 FORD Aspire, 19K mi, (first registr 12/96), 3 dr, 5 spd manual, dual air bags, still 14 mo or 17K mi factory warranty, $6,500, Clemens, X7249

MANUALS, complete shop, for `91-92, Saturn &nbsp, orig $190, will sell for $90, Frank, 531-3474


BERKELEY, beautiful, quiet, lg furn rm in brown shingle house (Julia Morgan), limited kitchen privileges, walk to campus, LBNL shuttle, BART, parks, downtown, ideal for visiting scholar, non-smoking, $450/mo, Rob, 843-5987

BERKELEY, 1 bdrm apt for sublet, Oct. through end of Feb. (possibly longer), located on Panoramic Way, behind Memorial Football Stadium, will be completely furnished, very cute, lower part of Berkeley hills, little yard, quiet & very safe area, looking for older single/couple responsible prospectives only, Chris, 849-4515

BERKELEY, sm 1 bdrm apt w/ spectacular view of bay on Panoramic Way, brand new, $1,075/month incl util, best for 1 person, Jacob, X4606

MONTCLAIR, Oakland Hills, 1 bdrm, 1 bth, private entrance, $875, Don or Emily, 339-2294


BEANIE babies, many retired, call for list and prices; great school-style wood bookcase for children's books, 4'Wx2'Dx 32"H, books covers are displayed, holds over 60 books, $40; wood & glass coffee table w/ matching all dark wood end tables, $300, Rick, X5882

BICYCLE, boy's red Schwinn Sidewinder, 20", exc cond, sold for $250 new, asking $100, Steve X4304, 631-0719

BICYCLES: women's 27", 12 spd, black Spalding Blade road bike; women's 26", 10 spd, blue Free Spirit Dynasty road bike, frnt & rear reflectors, owner's manual, both used very little, exc cond, $75 ea; Bell Windjammer bicycle helmets, 1 lg & 1 med, exc cond, $15 ea, Ron, X4410, 276-8079

COUCH, beige, exc cond, $250/b.o., redwood burl glass table, unique, $350, Lisa, X5314, (925) 906-9786

EXERCISE MACHINES: running machine, programmable w/ spd & incline, pulse monitor, Pro Form equalizer, 10.0si, $200/b.o; stair climber machine, programmable, electromagnetic drive, pulse monitor, Pro Form 3001, $200/b.o., Alastair X4276

GE WASHER & DRYER, full size, exc cond, $550; GE side by side refrig, exc cond, $870, Elina, X5017

GUITAR, Takamine EF-385, 12 string, acoustic/electric, built-in pre-amp, plays like an electric, include soft case, exc cond, $600/b.o.; amplifier Peavey Bandit 65, for guitar, 65 watts, 2 channel w/ reverb & distortion, $200/b.o., Tom, X6025, (707) 426-0717

KITCHEN UNIT, suitable for in-law unit or office, self-contained unit incl sink, 4 burner stove, oven, refrig/freezer, 52"x 27"x37", matching cabinets, 52"x27"x31" & overhead fan, copper color, $750/b.o., Margo X6280, (650) 871-4450

MOVING Sale, very lg navy blue rug, barely used, purchase price $210, selling for $100; 1 queen bed w/ frame, good cond, $100/ b.o.; 1 sofa bed, full size, good cond, $40/b.o.; 1 microwave oven, works well, $40/b.o., Mohammed, 807-3696 (pager)

NINETEENTH century American overshoot coverlet in red, white and blue colors (approx. size 72"x77"), very good cond, $400, Bev, (925) 933-6880

OPERA tickets, 1 pr for Peter Grimes, 12/11, frt row ctr of the grand tier, $240, Esther, X5306, 843-7678

OPERA tickets, 10/23 Manon; 10/30 Tristan und Isolde; 12/11 Peter Grimes, balc circle ctr, $150/pr, Diana, X6444

PASTA Machine, Popeil Deluxe, 24 dies, video & bonuses, brand new in box w/ orig $200 price tag, $75, Diana, X6444

PIANO, 1930 Baldwin Grand C 6-1/2', mahogany, glorious tone, $5,500. Mark, X7087 or 486-8079

ROTTWEILER pups, females, 16 wks and 2-1/2 yr old, both are black & mahogany, akc reg, $350 ea, Miguel, 237-1609

RUG, 9'x12' berber, brick color, $40, Herb, 232-0757

SPEAKERS, Acoustic Research model AR-3a, current retail $1,000/pair, refurb from orig owner for only $200, Martha, X4303, 548-7239

STROLLER, MacLaren twin side by side, $200, Peter, X7653

WEDDING DRESS & veil, never worn, ivory satin, size 7, Jessica McClintock, corset bodice w/ sweetheart neckline, long sleeves w/ pearl buttons, detachable/bustle able train, satin rosettes/bow at bustle, photo avail, $500 new, $250; ivory cake topper never used, still in box, $8; underwater camera, Nikon w/carrying case & instruction booklet, $40, Janice, X6412


HOUSE/APT, short-term house-sit/sublet, 12/19/98 to 1/2/99, prefer 2 bdrm house/apt in Berkeley, Emeryville, Albany, or SF area for visiting Swiss family, 1 couple, 1 adult, 3 children, Donald, 652-6434

HOUSING, research fellow seeking temporary housing nr LBNL fr Nov. 4 until Nov. 21, non-smoking, quiet, and responsible, email [email protected] or (314) 935-4898

HOUSE/CONDO, fully furn, 2+ bdrm, nr LBNL, for visiting Canadian scientist and wife, 1/1/99 to 4/30/99, Max, X4022

STUDIO or 1 bdrm apt in Berkeley, Oakland or SF, to sublet for the duration of 1998, for single prof person willing to house sit or lease, Eric, X6135

HEATER for in-house use, Christa, X7770

JAPANESE STN WAGON, 4 dr, manual transmission, late `80s or early `90s, Viki, 549-1876

MOVING MEN, two experienced guys to move UC staff member in Oakland within 3 mi radius, will pay $300 flat fee to move everything out, if you don't have a truck I'll rent U-haul, 10/17 or 10/24, Valerie, 642-4011

POOL TABLE, under $500, 3 pc slate, will arrange for moving, Rick, X5882

WATERBED, king, w/ headboard & 12 drawers underneath, decent cond, free (you haul from Lafayette w/ pickup truck), Joe, X5374


BAHAMAS CONDO, luxurious, spacious, 1 bdrm, in Taino Beach Resort, sleeps up to 4, every amenity, on beautiful beach, pool/tennis, maid service, $500/week, Marlene, X6005

HAWAII, 20 mi below Hilo on rainy side of Big Island, convenient to Univ of Hawaii campus & orchid plantations, 2 bdrm, 2 bth house for rent, unfurn, $450/mo or buy for $55,000, as is, nr schools, shopping, 1 mi to ocean bluff, Marlene, X6005

TAHOE KEYS at South Lake Tahoe, house, 3 bdrm 2 1/2 bth, on the water, fenced yard, quiet area close to many attractions, great views of water & mtns, $150/night, (2 night min), Bob, 925-376-2211


MATTRESS and springbox, twin size, working cond, you pick up, Chris, X6901


Flea Market Policy

Flea Market items may be e-mailed to fleamarket@, faxed to X6641, or mailed/delivered to Bldg. 65B. The deadline for the Oct. 23 issue is 5 p.m. Friday, Oct. 16. No ads will be accepted after that time.


Flea Market ad policy

Due to the large volume of ads received each week, ads are accepted only from LBNL employees, retirees, and on-site DOE personnel. No other ads will be accepted. We encourage past contributors to the Flea Market to use other local services, such as LBNL's online housing listing (call X6198 for information), and the UC Housing Office.

Please note also: